Search results for "ex situ conservation"

Genetic Distinctiveness Highlights the Conservation Value of a Sicilian Manna Ash Germplasm Collection Assigned to Fraxinus angustifolia (Oleaceae)

2020

The cosmopolitan genus Fraxinus comprises about 40 species occupying several habitats in the Northern Hemisphere. With some species hybridizing and sharing genetic variants, questions remain on the species assignment of germplasm within the genus Fraxinus despite numerous species-specific assessments. A multidisciplinary approach was employed to provide a definitive insight into the genetics of an endangered Fraxinus &ldquo

The “Sicilian Plant Germplasm Repository” of the University of Palermo: 25 years of activity in biological conservation

2018

The “Sicilian Plant Germplasm Repository” was created in 1993 from the Department of Botanical Sciences of the University of Palermo (SGCR/PA) – today section of Botany and Plant Ecology of the Department “STEBICEF” - with the aim to preserve the genetic diversity of endemic or endangered native plants, species of economical relevance and wild progenitors of plant cultivars. The collections are mostly constituted by seeds, and were recently expanded with tissues and DNA accessions. The specific tasks of SPGR/PA include the short- and long-term ex situ conservation and exchange of seeds, the recovery of the phytogenetic heritage, the reintroduction of threatened or endangered species into th…

The Genebank database of Palermo's Botanical Garden

2010

Is in vitro micrografting a possible valid alternative to traditional micropropagation in Cactaceae? Pelecyphora aselliformis as a case study

2016

Several taxa of Cactaceae are endangered by overcollection for commercial purposes, and most of the family is included in the Convention on International Trade in Endangered Species of Fauna and Flora (CITES). Micropropagation may play a key role to keep the pressure off wild populations and contribute to ex situ conservation of endangered taxa. One of the limits of micropropagation is the species-specific requirement of plant regulators for each taxon and sometimes even for different genotypes. With the micrografting technique the rootstock directly provides the scion with the necessary hormonal requirements. In this paper we present data on in vitro grafting of Pelecyphora aselliformis Eh…

IN-VITRO REGENERATION OF CALENDULA MARITIMA GUSS. (ASTERACEAE), A THREATENED PLANT ENDEMIC TO WESTERN SICILY

2016

Calendula maritima is a critically endangered endemic plant of Western Sicily. Besides habitat destruction, the hybridization with the contiguous congener species C. fulgida is a major threat to its conservation. For this reason, seed-based propagation and seed storage are not appropriate for conservation purposes. In the present paper we describe a rapid and prolific in vitro plant regeneration method by direct organogenesis from leaves of C. maritima. Leaf explants were cultured on solid Murashige and Skoog (MS) medium in the presence of several plant growth regulator combinations. The best shoot multiplication rate (2.5 shoots/explant) was obtained on the medium containing 4.4 µM 6-benzy…

Ex situ conservation of Sicilian endemic species in the Botanical garden of Catania University

2009

Genetic variations in the endangered Sicilian endemic Brassica rupestris: proposals for a conservation strategy

2012

Brassica rupestris Raf. is a chasmophyte species that includes two subspecies, both endemic to Central-Western Sicily (Italy). Inter-Simple Sequence Repeat (ISSR) markers were used to detect genetic diversity within and among eight populations representative of the species' distribution range. High levels of genetic diversity were revealed both at the population (PPB = 53.88%, HS = 0.212, Sh = 0.309) and at the species level (PPB = 96.55%, HT = 0.307, Sh = 0.464). The correlation between genetic and geographical distances was negative (Mantel test, r = -0.06, P < 0.95). The two subspecies of B. rupestris, subsp. rupestris and subsp. hispida, showed remarkable genetic similarity and molec…

Isozyme uniformity in a wild extinct insular plant,Lysimachia minoricensisJ.J. Rodr. (Primulaceae)

1999

Isozyme analyses were conducted to evaluate the genetic diversity of seed accessions of Lysimachia minoricensis (a Balearic endemic plant that became extinct in the wild) provided by 10 European botanical gardens. No isozyme variation was detected after examination of the electrophoretic patterns of 22 putative loci in more than 150 plants. The lack of genetic variation in L. minoricensis is probably due to the shortage of sample propagules originally recovered in the field before its extinction. Extant plants of L. minoricensis are believed to have originated from a single source, therefore limiting their use as seed stocks for restoration projects.

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

2023

The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient micropropagation protocol for Adenostyles by evaluating the effect of different plant growth regulators on leaf explants. Adenostyles alpina subsp. macrocephala (Asterace) is a perennial herbaceous plant endemic to Calabria (Southern Italy). The genus Adenostyles includes three species confined to the mountains of the Mediterranean and southern Europe. For callus induction, media supplemented with d…

Conservation priorities for harlequin frogs (Atelopus spp.) of Peru

2005

Populations of harlequin frogs Atelopus are declining throughout their geographical range. In Peru six of the 11 nominally described species are Critically Endangered, and there are at least 17 undescribed additional forms (all representing apparently distinct species), the conservation status of which is unknown. Most Atelopus taxa in Peru have relatively small geographical ranges, some known only from single populations, and have only a limited chance of survival. Conservation will require: (1) improvement of the faunistic and taxonomic knowledge of the genus, (2) field surveys, including in remote areas, (3) assessment of all species and populations for chytridiomycosis and research on t…